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a549 expressing h2b mruby  (ATCC)


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    ATCC a549 expressing h2b mruby
    A. Schematic of the screen. <t>A549</t> cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).
    A549 Expressing H2b Mruby, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 35644 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a549 expressing h2b mruby/product/ATCC
    Average 99 stars, based on 35644 article reviews
    a549 expressing h2b mruby - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Drug repurposing screen identifies masitinib as a 3CLpro inhibitor that blocks replication of SARS-CoV-2 in vitro"

    Article Title: Drug repurposing screen identifies masitinib as a 3CLpro inhibitor that blocks replication of SARS-CoV-2 in vitro

    Journal: bioRxiv

    doi: 10.1101/2020.08.31.274639

    A. Schematic of the screen. A549 cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).
    Figure Legend Snippet: A. Schematic of the screen. A549 cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).

    Techniques Used: Expressing, Infection, Incubation, Staining

    Of the 26 drugs that inhibited OC43 and tested against SARS-CoV-2, 20 inhibited SARS-CoV-2 replication in a dose-dependent manner, showing good concordance between OC43 and SARS-CoV-2 inhibition. A. A549 cells over-expressing ACE2 were pre-treated with indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and incubated for 2 days. Cells were stained for the presence of the spike protein and the % of infected cells was analyzed. Most of the drugs effective against OC43 showed similar effectivity against SARS-CoV-2, n=3. Individual measurements are shown as semi-transparent circles (note that some circles overlap). B. Effect of selected drugs on SARS-CoV-2 progeny production. Cells were treated with 10 μM of the indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and cell supernatant were collected for titration 2 days later, n = 3. Individual measurements are shown as semi-transparent circles. All drugs showed a statistically significant (p-values<0.001, one-tailed t-test, FDR-corrected) reduction in viral titers.
    Figure Legend Snippet: Of the 26 drugs that inhibited OC43 and tested against SARS-CoV-2, 20 inhibited SARS-CoV-2 replication in a dose-dependent manner, showing good concordance between OC43 and SARS-CoV-2 inhibition. A. A549 cells over-expressing ACE2 were pre-treated with indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and incubated for 2 days. Cells were stained for the presence of the spike protein and the % of infected cells was analyzed. Most of the drugs effective against OC43 showed similar effectivity against SARS-CoV-2, n=3. Individual measurements are shown as semi-transparent circles (note that some circles overlap). B. Effect of selected drugs on SARS-CoV-2 progeny production. Cells were treated with 10 μM of the indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and cell supernatant were collected for titration 2 days later, n = 3. Individual measurements are shown as semi-transparent circles. All drugs showed a statistically significant (p-values<0.001, one-tailed t-test, FDR-corrected) reduction in viral titers.

    Techniques Used: Inhibition, Expressing, Infection, Incubation, Staining, Titration, One-tailed Test



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    a549 expressing h2b mruby  (ATCC)
    99
    ATCC a549 expressing h2b mruby
    A. Schematic of the screen. <t>A549</t> cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).
    A549 Expressing H2b Mruby, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a549 expressing h2b mruby/product/ATCC
    Average 99 stars, based on 1 article reviews
    a549 expressing h2b mruby - by Bioz Stars, 2026-03
    99/100 stars
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    A. Schematic of the screen. A549 cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).

    Journal: bioRxiv

    Article Title: Drug repurposing screen identifies masitinib as a 3CLpro inhibitor that blocks replication of SARS-CoV-2 in vitro

    doi: 10.1101/2020.08.31.274639

    Figure Lengend Snippet: A. Schematic of the screen. A549 cells expressing H2B-mRuby were infected with OC43 (MOI 0.3), treated with drugs, incubated for 4 days at 33°C, and stained for the viral nucleoprotein. B. Screen results showing the %OC43 staining of mock-infected cells (green), no-drug controls (black), drugs with no effect on OC43 infection (blue), and screen hits (red). Overall agreement between the two repeats is high (R =0.81) C. Dose response curves of remdesivir and the top hits from the screen, n = 3. Individual measurements are shown as semi-transparent circles (note that some circles overlap).

    Article Snippet: A549 expressing H2B-mRuby were generated by first infecting A549 cells (ATCC CCL-185) with a lentivirus (carrying H2B-mRuby), and FACS-sorting mRuby+ cells.

    Techniques: Expressing, Infection, Incubation, Staining

    Of the 26 drugs that inhibited OC43 and tested against SARS-CoV-2, 20 inhibited SARS-CoV-2 replication in a dose-dependent manner, showing good concordance between OC43 and SARS-CoV-2 inhibition. A. A549 cells over-expressing ACE2 were pre-treated with indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and incubated for 2 days. Cells were stained for the presence of the spike protein and the % of infected cells was analyzed. Most of the drugs effective against OC43 showed similar effectivity against SARS-CoV-2, n=3. Individual measurements are shown as semi-transparent circles (note that some circles overlap). B. Effect of selected drugs on SARS-CoV-2 progeny production. Cells were treated with 10 μM of the indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and cell supernatant were collected for titration 2 days later, n = 3. Individual measurements are shown as semi-transparent circles. All drugs showed a statistically significant (p-values<0.001, one-tailed t-test, FDR-corrected) reduction in viral titers.

    Journal: bioRxiv

    Article Title: Drug repurposing screen identifies masitinib as a 3CLpro inhibitor that blocks replication of SARS-CoV-2 in vitro

    doi: 10.1101/2020.08.31.274639

    Figure Lengend Snippet: Of the 26 drugs that inhibited OC43 and tested against SARS-CoV-2, 20 inhibited SARS-CoV-2 replication in a dose-dependent manner, showing good concordance between OC43 and SARS-CoV-2 inhibition. A. A549 cells over-expressing ACE2 were pre-treated with indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and incubated for 2 days. Cells were stained for the presence of the spike protein and the % of infected cells was analyzed. Most of the drugs effective against OC43 showed similar effectivity against SARS-CoV-2, n=3. Individual measurements are shown as semi-transparent circles (note that some circles overlap). B. Effect of selected drugs on SARS-CoV-2 progeny production. Cells were treated with 10 μM of the indicated drugs for 2 hours, infected with SARS-CoV-2 (MOI 0.5) and cell supernatant were collected for titration 2 days later, n = 3. Individual measurements are shown as semi-transparent circles. All drugs showed a statistically significant (p-values<0.001, one-tailed t-test, FDR-corrected) reduction in viral titers.

    Article Snippet: A549 expressing H2B-mRuby were generated by first infecting A549 cells (ATCC CCL-185) with a lentivirus (carrying H2B-mRuby), and FACS-sorting mRuby+ cells.

    Techniques: Inhibition, Expressing, Infection, Incubation, Staining, Titration, One-tailed Test